Immobilization of lipase onto Cyperus Papyrus L. for biodiesel production by transesterification and hydrolysis-esterification

S Charuchinda, P Suthianthong, W Chulalaksananukul

Abstract


Lipase of Candida rugosa was immobilized on biomass support, plant fiber from Cyperus papyrus L., for biodiesel synthesis. The two immobilization techniques investigated in this study were physical adsorption and covalent binding with 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Results showed that the prepared immobilized lipase by physical adsorption with adding heptane presented higher protein loading, lipase activity and degree of immobilization than that by physical adsorption in phosphate buffer solution and by covalent binding. This immobilized lipase was further applied as biocatalyst for biodiesel synthesis by transesterification and hydrolysis-esterification. The results showed that this immobilized lipase preferred to hydrolyze triglyceride in palm oil to be fatty acid in water as medium. Then, the obtained fatty acid could be a good substrate to react with alcohol for biodiesel synthesis by esterification route. So, the biodiesel synthesis yield by hydrolysis-esterification was higher than transesterification. Nevertheless, the results revealed that bioethanol was found to be a better substrate than methanol for biodiesel synthesis via enzymatic hydrolysis-esterification.

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Printed ISSN: 0857-6149

Online ISSN: 2630-0508